Principle


The enzymatic hydrolysis of urea in aqueous solutions liberates
carbon dioxide and ammonia. The ions of these compounds increase
the conductivity of the solution.

Benefits


Compact, easily transportable setup

For both demonstration and student experiments

Flexible experimenting thanks to wireless data transmission

Additional experiment in plant physiology and biochemistry can be performed with few modifications

Data acquisition with tablets possible

With detailed experiment guide



Tasks


Conductivity measurements enable the rate of hydrolysis of urea
by the enzyme urease to be determined.

The addition of an appropriate inhibitor poisons the enzyme, so
that it no longer converts substrate.


What you can learn about


Poisoning of enzymes

Enzymatic hydrolysis of urea

Silver nitrate inhibition of urease

Conducivitiy measurement


Software included. Computer not provided.