Principle

In order to prevent nutrient media and cultures from being contaminated with microorganisms that adhere to the working equipment, the equipment, nutrient media, and nutrient solutions must be sterilised. Petri dishes filled with a solidified nutrient medium are called plates in the specialised language of microbiologists. The nutrient medium is poured into the dishes either from test tubes, with one test tube holding the required quantity of ready-made medium for one Petri dish, or from Erlenmeyer flasks if a large number of plates needs to be prepared at the same time.

Benefits


Experiment covers the basics of microbiology technics

Experiment is the first in a series of complementary microbiology experiments

Equipment of this experiment can also be used to perform the more advanced experiments


Tasks


Sterilisation of equipment

Preparation of standard nutrient agar for bacteria

Preparation of standard nutrient agar for moulds and yeasts

Preparation of a standard nutrient solution for bacteria

Preparation of slant agar tubes

Inoculation of microorganisms


What you can learn about


Basics of microbiological work techniques

Disinfection

Nutrient agar

Sterility

Sterilisation

Moulds

Yeast

Bacteria

Inoculation